Fa'aaliga Polotini Selele Iniseti
Ole faiga ole fa'aaliga ole iniseti ose fa'aoga eukaryotic e masani ona fa'aogaina mo le fa'aaliaina o polotini lapopo'a.Pe a faatusatusa i sela mamame, o tulaga o le aganuu o iniseti e faigofie ma e le manaʻomia CO2.O le Baculovirus o se ituaiga o siama DNA fa'alua fa'atasi ma sela iniseti e avea ma 'au masani.E maualuga lona tulaga fa'apitoa, e le afaina ai vetebrates, ma e le afaina i tagata ma lafumanu.sf9, o le mea e masani ona faʻaaogaina e fai ma cell host, e aliali mai i le planktonic poʻo le pipii i le aganuu.sf9 e fetaui lelei mo faʻamatalaga tetele, ma e mafai ona faʻaaogaina mo le gaioiga mulimuli ane ma suiga o polotini e pei o le phosphorylation, glycosylation, ma le acylation.E mafai fo'i ona fa'aoga le faiga o fa'amatalaga o le iniseti mo le fa'aalia o le tele o kenera, ma e mafai fo'i ona fa'aalia polotini oona e pei ole peptide antimicrobial.
Mea Au'aunaga | Taimi ta'ita'i(BD) |
Codon optimization, gene synthesis ma subcloning | 5-10 |
P1 fa'atupu fa'ama'i fa'ama'i ma fa'amatalaga la'ititi | 10-15 |
P2 generation virus incubation, fa'aaliga tele ma fa'amamāina, tu'uina atu o porotini mama ma lipoti fa'ata'ita'i |