Fa'amatalaga Fa'akomepiuta Selele Faafefete
O le fa'afefete fa'aaliga faiga o se auala fa'aaoga lautele mo le fa'aaliga o porotini eukaryotic, ona o lona faigofie i le fa'ato'aga, taugofie, ma le faigofie o le fa'aogaina.Faatasi ai ma ituaiga faafefete eseese, Pichia pastoris o le faʻaaliga sili ona lauiloa, aua e faʻafaigofie uma le faʻaaliga o le protein intracellular ma extracellular.E mafai fo'i e le faiga fa'aliliuga suiga, e pei o le phosphorylation ma le glycosylation, e maua ai se faiga fa'apitoa eukaryotic expression ma le tele o fa'amanuiaga.
Mea Au'aunaga | Taimi ta'ita'i(BD) |
Codon optimization, gene synthesis ma subcloning | 5-10 |
Su'esu'e lelei clone | 10-15 |
Fa'aaliga laititi | |
Lapo'a (200ML) fa'amatalaga ma fa'amamāina, fa'aolaina e aofia ai le porotini mama ma lipoti fa'ata'ita'i |
Afai e faʻapipiʻiina le kenele i Bioantibody, o le plasmid fausia o le a aofia i totonu o mea e mafai ona tuʻuina atu.